Force tuning through regulation of clathrin-dependent integrin endocytosis - Archive ouverte HAL Access content directly
Journal Articles Journal of Cell Biology Year : 2023

Force tuning through regulation of clathrin-dependent integrin endocytosis

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Anne-Pascale Bouin
Philippe Chavrier

Abstract

Integrin endocytosis is essential for many fundamental cellular processes. Whether and how the internalization impacts cellular mechanics remains elusive. Whereas previous studies reported the contribution of the integrin activator, talin, in force development, the involvement of inhibitors is less documented. We identified ICAP-1 as an integrin inhibitor involved in mechanotransduction by co-working with NME2 to control clathrin-mediated endocytosis of integrins at the edge of focal adhesions (FA). Loss of ICAP-1 enables β3-integrin-mediated force generation independently of β1 integrin. β3-integrin-mediated forces were associated with a decrease in β3 integrin dynamics stemming from their reduced diffusion within adhesion sites and slow turnover of FA. The decrease in β3 integrin dynamics correlated with a defect in integrin endocytosis. ICAP-1 acts as an adaptor for clathrin-dependent endocytosis of integrins. ICAP-1 controls integrin endocytosis by interacting with NME2, a key regulator of dynamin-dependent clathrin-coated pits fission. Control of clathrin-mediated integrin endocytosis by an inhibitor is an unprecedented mechanism to tune forces at FA.
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Dates and versions

hal-03834328 , version 1 (29-10-2022)

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Alexander Kyumurkov, Anne-Pascale Bouin, Mathieu Boissan, Sandra Manet, Francesco Baschieri, et al.. Force tuning through regulation of clathrin-dependent integrin endocytosis. Journal of Cell Biology, 2023, 222 (1), ⟨10.1083/jcb.202004025⟩. ⟨hal-03834328⟩
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