An experimental system to detect a labeled cell monolayer in a microfluidic environment - INRIA - Institut National de Recherche en Informatique et en Automatique Accéder directement au contenu
Article Dans Une Revue Journal of Magnetic Resonance Imaging Année : 2015

An experimental system to detect a labeled cell monolayer in a microfluidic environment

Résumé

This paper presents the feasibility of three-dimensional (3D) magnetic resonance (MR) histology of atheromatous coronary lesions in the entire human heart ex vivo using a standard 1.5-T scanner and a 12-mm high-temperature superconducting (HTS) surface coil. The HTS coil was a five-turn transmission-line resonator operated at 77 K, affording a signal-to-noise ratio (SNR) gain of about ninefold as compared to a similar, room-temperature copper coil. Local microscopy at the surface of an explanted, entire heart was achieved by a 3D spoiled gradient echo sequence and assessed by comparison with conventional histology. One hundred and twenty four adjacent cross sections of the coronary artery, with voxels of 59 × 59 × 100 µm 3 and an SNR of about 20, were obtained in 25 min. Consecutive data sets were combined to reconstruct extended views along the artery. Compared to histology, MR microscopy allowed precise nondestructive 3D depiction of the architecture of the atheromatous plaques. This is the first report of microscopic details (less than 10 −3 mm 3 voxels) of diseased arteries obtained in an entire human heart preserving the arterial integrity and the spatial geometry of atheroma. This noninvasive microscopy approach using a HTS surface coil might be applied in vivo to study the architecture and components of superficial human structures, using routine MR scanners.

Domaines

Autre
Fichier principal
Vignette du fichier
Main-Document-final (1).pdf (1.15 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02269942 , version 1 (23-08-2019)

Identifiants

Citer

Nicolas Gargam, Luc Darrasse, Jean-Sebastien Raynaud, Jean-Christoph Ginefri, Philippe Robert, et al.. An experimental system to detect a labeled cell monolayer in a microfluidic environment. Journal of Magnetic Resonance Imaging, 2015, 42 (4), pp.1100-1105. ⟨10.1002/jmri.24893⟩. ⟨hal-02269942⟩
33 Consultations
79 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More