Abstract : This paper proposes a one-shot six-channel multispectral color image acquisition system using a stereo camera and a pair of optical filters. The two filters from the best pair, selected from among readily available filters such that they modify the sensitivities of the two cameras in such a way that they produce optimal estimation of spectral reflectance and/or color, are placed in front of the two lenses of the stereo camera. The two images acquired from the stereo camera are then registered for pixel-to-pixel correspondence. The spectral reflectance and/or color at each pixel on the scene are estimated from the corresponding camera outputs in the two images. Both simulations and experiments have shown that the proposed system performs well both spectrally and colorimetrically. Since it acquires the multispectral images in one shot, the proposed system can solve the limitations of slow and complex acquisition process, and costliness of the state of the art multispectral imaging systems, leading to its possible uses in widespread applications.