Direct Sensing of Nutrients via a LAT1-like Transporter in Drosophila Insulin-Producing Cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cell Reports Année : 2016

Direct Sensing of Nutrients via a LAT1-like Transporter in Drosophila Insulin-Producing Cells

(1) , (2, 1) , (1) , (3) , (1)
1
2
3

Résumé

Dietary leucine has been suspected to play an important role in insulin release, a hormone that controls satiety and metabolism. The mechanism by which insulin-producing cells (IPCs) sense leucine and regulate insulin secretion is still poorly understood. In Drosophila, insulin-like peptides (DILP2 and DILP5) are produced by brain IPCs and are released in the hemolymph after leucine ingestion. Using Ca2+-imaging and ex vivo cultured larval brains, we demonstrate that IPCs can directly sense extracellular leucine levels via minidiscs (MND), a leucine transporter. MND knockdown in IPCs abolished leucine-dependent changes, including loss of DILP2 and DILP5 in IPC bodies, consistent with the idea that MND is necessary for leucine-dependent DILP release. This, in turn, leads to a strong increase in hemolymph sugar levels and reduced growth. GDH knockdown in IPCs also reduced leucine-dependent DILP release, suggesting that nutrient sensing is coupled to the glutamate dehydrogenase pathway.
Fichier principal
Vignette du fichier
maniere_2016_vol17_p137_cell_rep_{54174584-FCAE-41CD-9E50-778E654E21F9}.pdf (3.29 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01394085 , version 1 (26-09-2017)

Identifiants

Citer

Gérard Manière, Anna b. Ziegler, Flore Geillon, David e. Featherstone, Yael Grosjean. Direct Sensing of Nutrients via a LAT1-like Transporter in Drosophila Insulin-Producing Cells. Cell Reports, 2016, 17 (1), pp.137-148. ⟨10.1016/j.celrep.2016.08.093⟩. ⟨hal-01394085⟩
87 Consultations
144 Téléchargements

Altmetric

Partager

Gmail Facebook Twitter LinkedIn More