GipA Factor Supports Colonization of Peyerʼs Patches by Crohnʼs Disease-associated Escherichia Coli

Emilie Vazeille 1, 2 Benoit Chassaing 3 Anthony Buisson 2, 4 Anaëlle Dubois 2 Amélie de Vallée 2 Elisabeth Billard 2 Christel Neut 5 Gilles Bommelaer 2, 1 Jean-Frédéric Colombel 6 Nicolas Barnich 2 Arlette Darfeuille-Michaud 2 Marie-Agnès Bringer 7, 2, *
* Auteur correspondant
1 CHU Estaing [Clermont-Ferrand]
2 M2iSH - Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte
3 Georgia State University
4 CHU Clermont-Ferrand
5 Faculté des Sciences Pharmaceutiques et Biologiques
6 MSSM - Icahn School of Medicine at Mount Sinai [New York]
7 CSGA - Centre des Sciences du Goût et de l'Alimentation [Dijon]
Abstract : Background: Adherent-invasive Escherichia coli (AIEC) associated with Crohn's disease target M cells lining Peyer's patches (PPs) through the expression of long polar fimbriae (LPF) and survive macrophage killing. Invasion of PPs constitutes a way to colonize the mucosa for bacteria able to escape or resist killing of underlying immune cells. We aimed to identify new virulence factors involved in PPs colonization by AIEC. Methods: The presence of gipA (Growth in PPs) gene was determined by polymerase chain reaction. In vivo experiments were performed using CEABAC10 transgenic mice. Intramacrophagic behavior of AIEC was assessed in murine bone marrow-derived macrophages and human monocyte-derived macrophages. Cytokines production was quantified by ELISA. Results: A higher prevalence of gipA-positive E. coli was observed in patients with Crohn's disease (27.3%) compared with controls (17.2%). Unlike non-AIEC strains, all gipA-positive AIEC strains also harbored lpfA. GipA deletion impaired AIEC translocation across M cells and their replication inside macrophages. GipA expression was induced by gastrointestinal (bile salts) and phagolysosomal (reactive oxygen species and acid pH) conditions. GipA deletion decreased lpfA mRNA level in AIEC bacteria. Survival of AIEC-Delta gipA bacteria was reduced in medium containing H2O2 or acidic pH. GipA deletion impaired AIEC colonization of PPs and dissemination to mesenteric lymph nodes in mice. Conclusions: GipA is required for optimal colonization of mouse PPs and survival within macrophages by AIEC, suggesting that this factor plays a role in AIEC promotion of Crohn's disease. Detection of gipA and lpfA could be a predictor for the presence of AIEC.
Keywords : GipA adherent-invasive Escherichia coli Peyer's patches Crohn's disease invasive e. coli follicle-associated epithelium inflammatory-bowel-disease m-cells ileal mucosa strain lf82 bacterial translocation intestinal microbiota shigella-flexneri genome sequence
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01396555
Contributeur : Csga - Université de Bourgogne <>
Soumis le : lundi 14 novembre 2016 - 15:52:04
Dernière modification le : vendredi 8 novembre 2019 - 10:55:22

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Emilie Vazeille, Benoit Chassaing, Anthony Buisson, Anaëlle Dubois, Amélie de Vallée, et al.. GipA Factor Supports Colonization of Peyerʼs Patches by Crohnʼs Disease-associated Escherichia Coli. Inflammatory Bowel Diseases, Lippincott, Williams & Wilkins, 2016, 22 (1), pp.68 - 81. ⟨10.1097/MIB.0000000000000609⟩. ⟨hal-01396555⟩

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