Biofilms of Lactobacillus plantarum and Lactobacillus fermentum: effect on stress responses, antagonistic effects on pathogen growth and immunomodulatory properties

Nabil Aoudia 1 Aurélie Rieu 1, * Romain Briandet 2 Julien Deschamps 2 Johanna Chluba 3, 4, 5 Gaëtan Jego 4, 5 Carmen Garrido 4, 6, 5, 3 Jean Guzzo 1
* Auteur correspondant
1 PAM - Procédés Alimentaires et Microbiologiques
2 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
3 LabEx LipSTIC - Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer
4 LCE - Laboratoire Chrono-environnement - UFC (UMR 6249)
5 NUTox - Physiologie de la Nutrition et Toxicologie (NUTox) (U866, Lipides et nutrition, équipe 7)
LNC - Lipides - Nutrition - Cancer (U866)
6 CRLCC - CGFL - Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc
Abstract : Few studies have extensively investigated probiotic functions associated with biofilms. Here, we show that strains of Lactobacillus plantarum and Lactobacillus fermentum are able to grow as biofilm on abiotic surfaces, but the biomass density differs between strains. We performed microtiter plate biofilm assays under growth conditions mimicking to the gastrointestinal environment. Osmolarity and low concentrations of bile significantly enhanced Lactobacillus spatial organization. Two L. plantarum strains were able to form biofilms under high concentrations of bile and mucus. We used the agar well-diffusion method to show that supernatants from all Lactobacillus except the NA4 isolate produced food pathogen inhibitory molecules in biofilm. Moreover, TNF-alpha production by LPS-activated human monocytoid cells was suppressed by supernatants from Lactobacillus cultivated as biofilms but not by planktonic culture supernatants. However, only L. fermentum NA4 showed anti-inflammatory effects in zebrafish embryos fed with probiotic bacteria, as assessed by cytokine transcript level (TNF-alpha, IL-1 beta and IL-10). We conclude that the biofilm mode of life is associated with beneficial probiotic properties of lactobacilli, in a strain dependent manner. Those results suggest that characterization of isolate phenotype in the biofilm state could be additional valuable information for the selection of probiotic strains.
Keywords : Communities Resistance In-vitro Sulfonic-acid Lactic-acid bacteria Pseudomonas-aeruginosa biofilms Zebrafish model Vibrio-cholerae Bile-salt hydrolase Probiotic bacteria Escherichia-coli Lactobacillus biofilms Probiotic Immunomodulatory effects
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01432554
Contributeur : Pam - Université de Bourgogne <>
Soumis le : mercredi 11 janvier 2017 - 17:48:40
Dernière modification le : mercredi 8 janvier 2020 - 14:20:05

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UNIV-BOURGOGNE | INRA | CGFL | FNCLCC | PAM | AGROPARISTECH | CNRS | UNIV-FCOMTE | EPHE | PSL | LNC-UMR866 | UNIV-PARIS-SACLAY | LNC-HSPPATHIES | CHRONO-ENVIRONNEMENT | ENSBANA | AGROSUP-DIJON | UMR866-EQUIPE7 | UNIV-MONTPELLIER

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Nabil Aoudia, Aurélie Rieu, Romain Briandet, Julien Deschamps, Johanna Chluba, et al.. Biofilms of Lactobacillus plantarum and Lactobacillus fermentum: effect on stress responses, antagonistic effects on pathogen growth and immunomodulatory properties. Food Microbiology, Elsevier, 2016, 53, pp.51 - 59. ⟨10.1016/j.fm.2015.04.009⟩. ⟨hal-01432554⟩

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