The impact of tumor nitric oxide production on VEGFA expression and tumor growth in a zebrafish rat glioma xenograft model. - Université de Bourgogne Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2015

The impact of tumor nitric oxide production on VEGFA expression and tumor growth in a zebrafish rat glioma xenograft model.

Impact de la production de monoxyde d'azote par la tumeur sur l'expression de VEGFA et la croissance tumorale dans un modèle de xénogreffe de gliome de rat chez le poisson zèbre

Eric Solary

Résumé

To investigate the effect of nitric oxide on tumor development, we established a rat tumor xenograft model in zebrafish embryos. The injected tumor cells formed masses in which nitric oxide production could be detected by the use of the cell-permeant DAF-FM-DA (diaminofluorophore 4-amino-5-methylamino-2'-7'-difluorofluorescein diacetate) and DAR-4M-AM (diaminorhodamine-4M). This method revealed that nitric oxide production could be co-localized with the tumor xenograft in 46% of the embryos. In 85% of these embryos, tumors were vascularized and blood vessels were observed on day 4 post injection. Furthermore, we demonstrated by qRT-PCR that the transplanted glioma cells highly expressed Nos2, Vegfa and Cyclin D1 mRNA. In the xenografted embryos we also found increased zebrafish vegfa expression. Glioma and zebrafish derived Vegfa and tumor Cyclin D1 expression could be down regulated by the nitric oxide scavenger 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide or CPTIO. We conclude that even if there is a heterogeneous nitric oxide production by the xenografted glioma cells that impacts Vegfa and Cyclin D1 expression levels, our results suggest that reduction of nitric oxide levels by nitric oxide scavenging could be an efficient approach to treat glioma.
Fichier principal
Vignette du fichier
3ec4d33ffe7b390316456ee04b617ae9.pdf (1.25 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-01446713 , version 1 (05-02-2024)

Identifiants

Citer

Nadhir Yousfi, Benoist Pruvot, Tatiana Lopez, Lea Magadoux, Nathalie Franche, et al.. The impact of tumor nitric oxide production on VEGFA expression and tumor growth in a zebrafish rat glioma xenograft model.. PLoS ONE, 2015, 10 (3), pp.e0120435. ⟨10.1371/journal.pone.0120435⟩. ⟨hal-01446713⟩
217 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More