Enterocyte Purge and Rapid Recovery Is a Resilience Reaction of the Gut Epithelium to Pore-Forming Toxin Attack

Kwang-Zin Lee; Matthieu Lestradet; Catherine Socha; Stefanie Schirmeier; Antonin Schmitz; Caroline Spenlé; Olivier Lefebvre; Céline Keime; Wennida M. Yamba; Richard Bou Aoun; Samuel Liegeois; Yannick Schwab; Patricia Simon-Assmann; Frédéric Dalle; Dominique Ferrandon

doi:10.1016/j.chom.2016.10.010

Article dans une revue

Enterocyte Purge and Rapid Recovery Is a Resilience Reaction of the Gut Epithelium to Pore-Forming Toxin Attack

Kwang-Zin Lee ^{1, 2, *} Matthieu Lestradet ^{3, 1} Catherine Socha ¹ Stefanie Schirmeier ^{1, 4} Antonin Schmitz ⁵ Caroline Spenlé ^{6, 7, 8, 9} Olivier Lefebvre ^{6, 7, 8, 9} Céline Keime ¹⁰ Wennida M. Yamba ¹ Richard Bou Aoun ^{1, 11} Samuel Liegeois ¹ Yannick Schwab ¹⁰ Patricia Simon-Assmann ^{6, 7, 8, 9} Frédéric Dalle ^{12, 5} Dominique Ferrandon ^{1, *}

* Auteur correspondant

1 RIDI - UPR 9002 - Réponse immunitaire et developpement chez les insectes

2 Institut für Insektenbiotechnologie [Justus-Liebig-Universität Gießen]

3 INMG - Institut NeuroMyoGène

4 Institut für Neuro- und Verhaltensbiologie [Münster]

5 Agroécologie [Dijon]

6 Immuno-Rhumatologie Moléculaire

7 UNISTRA - Université de Strasbourg

8 FMTS - Fédération de Médecine Translationnelle de Strasbourg

9 Centre de recherche du médicament Medalis - Drug Discovery Center [LabEx]

10 IGBMC - Institut de génétique et biologie moléculaire et cellulaire

11 CEED - Defymed : Centre Européen Étude du Diabète

12 PAM - Procédés Alimentaires et Microbiologiques

Abstract : Besides digesting nutrients, the gut protects the host against invasion by pathogens. Enterocytes may be subjected to damage by both microbial and host defensive responses, causing their death. Here, we report a rapid epithelial response that alleviates infection stress and protects the enterocytes from the action of microbial virulence factors. Intestinal epithelia exposed to hemolysin, a pore-forming toxin secreted by Serratia marcescens, undergo an evolutionarily conserved process of thinning followed by the recovery of their initial thickness within a few hours. In response to hemolysin attack, Drosophila melanogaster enterocytes extrude most of their apical cytoplasm, including damaged organelles such as mitochondria, yet do not lyse. We identify two secreted peptides, the expression of which requires CyclinJ, that mediate the recovery phase in which enterocytes regain their original shape and volume. Epithelial thinning and recovery constitute a fast and efficient response to intestinal infections, with pore-forming toxins acting as alarm signals.

Keywords : Drosophila cyclin j Mitochondrial extrusion Bacterial-infection Serratia-marcescens Disease tolerance Virulence factors Defense strategy Plasma-membrane Lipid droplets Oral infection

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio] / Ingénierie des aliments

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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01507264
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Soumis le : mercredi 12 avril 2017 - 17:58:33
Dernière modification le : jeudi 17 juin 2021 - 15:16:03

Enterocyte Purge and Rapid Recovery Is a Resilience Reaction of the Gut Epithelium to Pore-Forming Toxin Attack

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