Redshifted Cherenkov Radiation for in vivo Imaging: Coupling Cherenkov Radiation Energy Transfer to multiple Förster Resonance Energy Transfers

Abstract : Cherenkov Radiation (CR), this blue glow seen in nuclear reactors, is an optical light originating from energetic beta-emitter radionuclides. CR emitter Y-90 triggers a cascade of energy transfers in the presence of a mixed population of fluorophores (which each other match their respective absorption and emission maxima): Cherenkov Radiation Energy Transfer (CRET) first, followed by multiple Forster Resonance Energy transfers (FRET): CRET ratios were calculated to give a rough estimate of the transfer efficiency. While CR is blue-weighted (300-500 nm), such cascades of Energy Transfers allowed to get a) fluorescence emission up to 710 nm, which is beyond the main CR window and within the near-infrared (NIR) window where biological tissues are most transparent, b) to amplify this emission and boost the radiance on that window: EMT6-tumor bearing mice injected with both a radionuclide and a mixture of fluorophores having a good spectral overlap, were shown to have nearly a two-fold radiance boost (measured on a NIR window centered on the emission wavelength of the last fluorophore in the Energy Transfer cascade) compared to a tumor injected with the radionuclide only. Some CR embarked light source could be converted into a near-infrared radiation, where biological tissues are most transparent.
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Scientific Reports, Nature Publishing Group, 2017, 7 (45036), 〈https://www.nature.com/articles/srep45063〉. 〈10.1038/srep45063〉
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01522064
Contributeur : Icmub - Université de Bourgogne <>
Soumis le : vendredi 12 mai 2017 - 17:20:46
Dernière modification le : vendredi 23 février 2018 - 14:30:02

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Yann Bernhard, Bertrand Collin, Richard A. Decréau. Redshifted Cherenkov Radiation for in vivo Imaging: Coupling Cherenkov Radiation Energy Transfer to multiple Förster Resonance Energy Transfers. Scientific Reports, Nature Publishing Group, 2017, 7 (45036), 〈https://www.nature.com/articles/srep45063〉. 〈10.1038/srep45063〉. 〈hal-01522064〉

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