Carob leaf polyphenols trigger intrinsic apoptotic pathway and induce cell cycle arrest in colon cancer cells

Fatima Zahra Ghanemi; Meriem Belarbi; Aurélie Fluckiger; Abdelhafid Nani; Adelie Dumont; Charlotte de Rosny; Ikram Aboura; Amira Sayed Khan; Babar Murtaza; Chahid Benammar; Boucif Farid Lahfa; Danish Patoli; Dominique Delmas; Cédric Rébé; Lionel Apétoh; Naim Akhtar Khan; François Ghringhelli; Mickael Rialland; Aziz Hichami

doi:10.1016/j.jff.2017.03.032

Carob leaf polyphenols trigger intrinsic apoptotic pathway and induce cell cycle arrest in colon cancer cells

Fatima Zahra Ghanemi ¹ Meriem Belarbi ¹ Aurélie Fluckiger Abdelhafid Nani ^{2, *} Adelie Dumont Charlotte de Rosny Ikram Aboura ¹ Amira Sayed Khan ³ Babar Murtaza ⁴ Chahid Benammar ¹ Boucif Farid Lahfa Danish Patoli Dominique Delmas ⁵ Cédric Rébé ⁵ Lionel Apétoh ⁶ Naim Akhtar Khan ⁷ François Ghringhelli ^{8, 5} Mickael Rialland Aziz Hichami ^*

* Auteur correspondant

1 Université Aboubekr Belkaid - University of Belkaïd Abou Bekr [Tlemcen]

2 Service d'Hépato-gastro-entérologie [CHRU Nancy]

3 LNC - Lipides - Nutrition - Cancer [Dijon - U1231]

4 NUTox - Physiologie de la Nutrition et Toxicologie (NUTox) (U866, Lipides et nutrition, équipe 7)

LNC - Lipides - Nutrition - Cancer (U866)

5 Equipe CADIR (LNC - U1231)

LNC - Lipides - Nutrition - Cancer [Dijon - U1231]

6 Equipe CD4DNA (LNC - U1231)

LNC - Lipides - Nutrition - Cancer [Dijon - U1231]

7 NUTOX - Equipe NuTox (LNC - U1231)

LNC - Lipides - Nutrition - Cancer [Dijon - U1231]

8 CRLCC - CGFL - Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc

Abstract : Chemoprevention of Colorectal cancer (CRC) is the major concern for improving public health. We investigated the protective effects of carob, Ceratonia siliqua L, leaf polyphenols (CLP) against CRC. Phenolic content analysis showed that CLP is enriched with gallic acid and m-coumaric acid. We observed that CLP exerted a dose dependent cytotoxic effect through the induction of apoptosis on CRC cell lines, with an IC50 around 20 mu g/mL CLP induced intrinsic apoptotic pathway through the caspase-9 activation and PARP cleavage in HCT-116 and CT-26 cells. Moreover, CLP induced cell cycle arrest in the G1 phase through p53 activation. Gallic acid and m-coumaric acid exerted similar effect of CLP but at higher concentration to that expected to be present in CLP, suggesting the synergistic effect between polyphenols in CLP activity. Interestingly, the carob leaf infusion reduced CT-26 tumour growth in BALB/c mice. This study suggests that CLP can be used for the prevention of CRC. (C) 2017 Elsevier Ltd. All rights reserved.

Keywords : Cell cycle arrest Carob (Ceratonia siliqua L.) Polyphenols Colorectal cancer Caspases Apoptosis

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio] / Alimentation et Nutrition

Liste complète des métadonnées

https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01536927
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : lundi 12 juin 2017 - 11:55:53
Dernière modification le : vendredi 9 novembre 2018 - 14:52:02

Carob leaf polyphenols trigger intrinsic apoptotic pathway and induce cell cycle arrest in colon cancer cells

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