Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8

Abstract : Analysis of CD95/Fas complexes by immunoprecipitation has long relied on the monoclonal antibody APO1 or tagged recombinant Fas ligand. Immunoprecipitation is an elegant and efficient procedure to investigate endogenous protein interactions or complexes. Provided that the targeted complex is soluble in mild detergent these complexes can be recovered using protein A/G-coupled Sepharose beads and further analyzed after denaturation and electrophoretic separation by western blotting or mass spectrometry. Herein, we describe in detail the method used in our laboratory to immunoprecipitate and analyze by immunoblot complexes containing caspase-8, using a commercial antibody directed against caspase-8.
Type de document :
Chapitre d'ouvrage
Springer Protocols. CD 95 : Methods and Protocols, 1557, Humana Press, pp.19-31, 2017, 978-1493967780. 〈10.​1007/​978-1-4939-6780-3_​22〉. 〈https://link.springer.com/protocol/10.1007%2F978-1-4939-6780-3_3〉
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01548294
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : mardi 27 juin 2017 - 13:55:08
Dernière modification le : vendredi 8 juin 2018 - 14:50:25

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Andrei Alexandru Constantinescu, Aymeric Morlé, Olivier Micheau. Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8. Springer Protocols. CD 95 : Methods and Protocols, 1557, Humana Press, pp.19-31, 2017, 978-1493967780. 〈10.​1007/​978-1-4939-6780-3_​22〉. 〈https://link.springer.com/protocol/10.1007%2F978-1-4939-6780-3_3〉. 〈hal-01548294〉

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