Sleeve Gastrectomy Alters Intestinal Permeability in Diet-Induced Obese Mice

Résumé : BACKGROUND: Increased lipopolysaccharide (LPS) translocation due to altered intestinal permeability has been suggested as a mechanism for obesity-associated insulin resistance. The goal of this study was to assess the effect of sleeve gastrectomy (SG) on intestinal barrier permeability in diet-induced obese mice. MATERIALS AND METHODS: Four weeks after surgery, the effects of SG on intestinal permeabilities were assessed ex vivo and in vivo in male C57Bl/6J mice fed a high-fat diet. Gene expression of tight junction proteins and inflammatory cytokines was measured in jejunum, colon, liver, and inguinal adipose tissue. Plasma LPS was quantified by HPLCMS/MS spectrometry. RESULTS: SG significantly reduced body weight and improved glucose homeostasis, as expected. SG decreased paracellular (p = 0.01) and transcellular permeability (p = 0.03) in the jejunum; and increased mRNA levels of the tight junction proteins Jam A (p = 0.02) and occludin (p = 0.01). In contrast in the distal colon, paracellular permeability tended to be increased (p = 0.07) while transcellular permeability was significantly induced (p = 0.03) after SG. In vivo, the paracellular permeability was significantly increased 3 weeks after SG (p = 0.02). Plasma LPS level were increased after SG (p = 0.03), as well as mRNA levels of adipose and hepatic inflammatory markers (p = 0.02). CONCLUSIONS: SG significantly modifies intestinal permeability in a differential manner between the proximal and distal intestine. These changes promote LPS translocation in plasma, induce a low-grade pro-inflammatory state in adipose tissue and liver, but do not impair the SG-induced glucose homeostasis improvement.
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Contributeur : Lnc - Université de Bourgogne <>
Soumis le : mardi 11 juillet 2017 - 15:50:39
Dernière modification le : vendredi 8 juin 2018 - 14:50:25



Claire Blanchard, François Moreau, Julien Chevalier, Audrey Ayer, Damien Garcon, et al.. Sleeve Gastrectomy Alters Intestinal Permeability in Diet-Induced Obese Mice. Obesity Surgery, Springer Verlag, 2017, pp.1-9. 〈〉. 〈10.1007/s11695-017-2670-1〉. 〈hal-01560488〉



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