A Biocatalytic Nanomaterial for the Label-Free Detection of Virus-Like Particles

Abstract : The design of nanomaterials that are capable of specific and sensitive biomolecular recognition is an on-going challenge in the chemical and biochemical sciences. A number of sophisticated artificial systems have been designed to specifically recognize a variety of targets. However, methods based on natural biomolecular detection systems using antibodies are often superior. Besides greater affinity and selectivity, antibodies can be easily coupled to enzymatic systems that act as signal amplifiers, thus permitting impressively low detection limits. The possibility to translate this concept to artificial recognition systems remains limited due to design incompatibilities. Here we describe the synthesis of a synthetic nanomaterial capable of specific biomolecular detection by using an internal biocatalytic colorimetric detection and amplification system. The design of this nanomaterial relies on the ability to accurately grow hybrid protein-organosilica layers at the surface of silica nanoparticles. The method allows for label-free detection and quantification of targets at picomolar concentrations.The design of nanomaterials that are capable of specific and sensitive biomolecular recognition is an on-going challenge in the chemical and biochemical sciences. A number of sophisticated artificial systems have been designed to specifically recognize a variety of targets. However, methods based on natural biomolecular detection systems using antibodies are often superior. Besides greater affinity and selectivity, antibodies can be easily coupled to enzymatic systems that act as signal amplifiers, thus permitting impressively low detection limits. The possibility to translate this concept to artificial recognition systems remains limited due to design incompatibilities. Here we describe the synthesis of a synthetic nanomaterial capable of specific biomolecular detection by using an internal biocatalytic colorimetric detection and amplification system. The design of this nanomaterial relies on the ability to accurately grow hybrid protein-organosilica layers at the surface of silica nanoparticles. The method allows for label-free detection and quantification of targets at picomolar concentrations.
Type de document :
Article dans une revue
ChemBioChem, Wiley-VCH Verlag, 2017, 18 (11), pp.996 - 1000. 〈10.1002/cbic.201700126〉
Liste complète des métadonnées

https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01560577
Contributeur : Pam - Université de Bourgogne <>
Soumis le : mardi 11 juillet 2017 - 16:40:22
Dernière modification le : mercredi 5 septembre 2018 - 17:04:05

Identifiants

Collections

PAM

Citation

Sabine Sykora, M. Rita Correro, Negar Moridi, Gaël Belliot, Pierre Pothier, et al.. A Biocatalytic Nanomaterial for the Label-Free Detection of Virus-Like Particles. ChemBioChem, Wiley-VCH Verlag, 2017, 18 (11), pp.996 - 1000. 〈10.1002/cbic.201700126〉. 〈hal-01560577〉

Partager

Métriques

Consultations de la notice

36