In vitro and in vivo trackable titanocene-based complexes using optical imaging or SPECT

Océane Florès (1) , Audrey Trommenschlager (1) , Souheila Amor (1) , Fernanda Marques (2) , Francisco Silva (2) , Lurdes Gano (2) , Franck Denat (1) , Maria Paula Cabral Campello (2) , Christine Goze (1) , Ewen Bodio (1) , Pierre Le Gendre (1)

1 ICMUB - Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon]
2 Centro de Ciencias e Tecnologias Nucleares, Instituto Superior Tecnico, Universidade de Lisboa, Bobadela LRS, Portugal

A novel Ti/111In-heterometallic radiotheranostic along with non-radioactive Ti/In, Ti/Lu, and Ti/Y analogues has been reported, thanks to the design of a challenging synthesis of the first titanocene-DOTA ligand. The corresponding titanocene-BODIPY complex was developed for in vitro tracking by optical imaging. The different complexes were characterized and their antiproliferative properties were evaluated on three cancer cell lines (A2780, B16F1, and PC3). As a proof of concept, initial studies in healthy mice were performed with a Ti/111In derivative to obtain information about its uptake, its biodistribution, and its excretion. Confocal microscopy experiments were performed with fluorescent complexes to track it in vitro.

Domaines: Chimie

Océane Florès, Audrey Trommenschlager, Souheila Amor, Fernanda Marques, Francisco Silva, et al.. In vitro and in vivo trackable titanocene-based complexes using optical imaging or SPECT. Dalton Transactions, 2017, ⟨10.1039/C7DT01981E⟩. ⟨hal-01566449⟩