Marine actinomycete crude extracts with potent TRAIL-resistance overcoming activity against breast cancer cells.

Abstract : Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent, as it can kill tumor cells selectively. In our search of bioactive natural products to overcome TRAIL-resistance, we isolated 47 actinomycete strains from different sediments and seawater samples collected from the Red Sea coast in Egypt and found four crude extracts (EGY1, EGY3, EGY24 and EGY34) displaying TRAIL sensitizing activity in the resistant breast cancer cell line MDA-MB-231. None of these crude extracts exhibited cytotoxic effect on normal mouse embryonic fibroblasts (MEF), with the exception of EGY34. Analysis of the signaling pathways underlying the sensitization of MDA-MB-231 cells to TRAIL-induced apoptosis, by western blotting, revealed that all crude extracts facilitated initiator caspase‑8/-10 activation upon TRAIL stimulation, but that in addition, EGY3 and EGY34, alone, induced strong ER-stress activation, with the appearance of BiP in the cytosolic extracts. Our results pave the way to the discovery and the development of marine-derived drugs for cancer therapy.
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Oncology Reports, Spandidos Publications, 2017, 37 (6), pp.3635-3642. 〈https://www.spandidos-publications.com/or/37/6/3635?text=fulltext〉. 〈10.3892/or.2017.5595〉
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01576817
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : jeudi 24 août 2017 - 09:51:56
Dernière modification le : vendredi 8 juin 2018 - 14:50:25

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Mohammed I Y Elmallah, Olivier Micheau, Mennat Allah G Eid, Ali M S Hebishy, Mohamed S Abdelfattah. Marine actinomycete crude extracts with potent TRAIL-resistance overcoming activity against breast cancer cells.. Oncology Reports, Spandidos Publications, 2017, 37 (6), pp.3635-3642. 〈https://www.spandidos-publications.com/or/37/6/3635?text=fulltext〉. 〈10.3892/or.2017.5595〉. 〈hal-01576817〉

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