An siRNA-based screen in C2C12 myoblasts identifies novel genes involved in myogenic differentiation

Rayan Alwan 1 Ange-Line Bruel 2 Anne da Silva 1 Véronique Blanquet 1, * Khaled Bouhouche 1
* Auteur correspondant
1 UGMA - Unité de Génétique Moléculaire Animale
2 Equipe GAD (LNC - U1231)
LNC - Lipides - Nutrition - Cancer [Dijon - U1231]
Abstract : Myogenesis is a highly regulated multi-step process involving myoblast proliferation and differentiation. Although studies over the last decades have identified several factors governing these distinct major phases, many of them are not yet known. In order to identify novel genes, we took advantage of the C2C12 myoblastic line to establish a functional siRNA screen combined with quantitative-imaging analysis of a large amount of differentiated myoblasts. We knocked down 100 preselected mouse genes without a previously characterized role in muscle. Using image analysis, we tracked gene-silencing phenotypes by quantitative assessment of cellular density, myotube quantity, myotube morphology and fusion index. Our results have revealed six genes involved in both stages of C2C12 myogenesis and 13 genes specific to the differentiation stage. These findings prove that our RNAi-based screen could be an efficient tool to detect clear and subtle phenotypes allowing the identification of new myogenic regulators in mammals.
Keywords : Screen siRNA C2C12 Proliferation Differentiation Myogenesis
Type de document :
Article dans une revue
Liste complète des métadonnées
https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01599315
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : lundi 2 octobre 2017 - 09:58:03
Dernière modification le : lundi 24 septembre 2018 - 09:42:22

Identifiants

Collections

UNIV-BOURGOGNE | INRA | UNILIM | LNC-UMR866 | LNC-GAD | PEIRENE | GEIST

Citation

Rayan Alwan, Ange-Line Bruel, Anne da Silva, Véronique Blanquet, Khaled Bouhouche. An siRNA-based screen in C2C12 myoblasts identifies novel genes involved in myogenic differentiation. Experimental Cell Research, Elsevier, 2017, 359 (1), pp.145-153. ⟨10.1016/j.yexcr.2017.07.037⟩. ⟨hal-01599315⟩

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

114

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD