Viral prevalence and laboratory investigations of gastroenteritis in institutions for dependent people

Abstract : OBJECTIVES: Viruses are recognized as important agents of gastroenteritis outbreaks in institutions caring for dependent people. We aimed to define norovirus (NoV) and rotavirus (RV) immunochromatographic tests (ICT) and reverse transcription-polymerase chain reaction (RT-PCR) used in gastroenteritis investigations. METHODS: Fourteen sites were monitored from 2010 to 2015, with 360 laboratory investigations. Twenty-two outbreaks and 44 sporadic case patients were investigated with ICTs (114 NoVs and 80 RVs) and RT-PCRs (114 NoVs and 52 RVs). RESULTS: ICTs were useful during outbreaks (identification of NoVs and RVs in respectively 76.5% and 75.0% of episodes) despite the NoV sensitivity limit (55.1%) and the four RV false positive results observed for 10 samples. Given the NoV ICT performance and the observed variations of the NoV and RV prevalence (between 20.0% and 5.0%), ICTs are not appropriate to identify sporadic gastroenteritis case patients. Positive predictive values <60.0% were observed when the prevalence of RV and NoV was low (<5.0%). NoV and RV RT-PCR indications are sporadic gastroenteritis case patients, negative NoV and RV ICT during outbreaks, control of positive RV ICT in cases of suspected NoV and RV co-circulation, patients with long symptom duration, and NoV genogroup and genotype identifications (infection control and epidemiological surveillance). Inclusion of patients with specific clinical symptoms is recommended irrespective of the technique. CONCLUSION: On the basis of the ICT limits identified in this work, RT-PCR development seems essential to improve viral gastroenteritis investigations in institutions caring for dependent people.
Type de document :
Article dans une revue
Médecine et Maladies Infectieuses, Elsevier Masson, 2017, 〈10.1016/j.medmal.2017.09.007〉
Liste complète des métadonnées

https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01620013
Contributeur : Pam - Université de Bourgogne <>
Soumis le : vendredi 20 octobre 2017 - 09:14:17
Dernière modification le : mercredi 5 septembre 2018 - 17:04:05

Identifiants

Citation

P. Gaspard, P. Pothier, C. Roth, S. Larocca, B. Heck, et al.. Viral prevalence and laboratory investigations of gastroenteritis in institutions for dependent people. Médecine et Maladies Infectieuses, Elsevier Masson, 2017, 〈10.1016/j.medmal.2017.09.007〉. 〈hal-01620013〉

Partager

Métriques

Consultations de la notice

62