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Genotoxic effects of food contact recycled paperboard extracts on two human hepatic cell lines.

Emilie Souton 1 Isabelle Séverin 1 Ludovic Le Hegarat 2 Kevin Hogeveen 2 Abdulhadi Aljawish 1 Valérie Fessard 2 Marie-Christine Chagnon 1, * 
* Auteur correspondant
1 NUTOX - Equipe NuTox (LNC - U1231)
LNC - Lipides - Nutrition - Cancer [Dijon - U1231]
2 Unité de Toxicologie des Contaminants
Laboratoire de Fougères - ANSES
Abstract : Food contact paperboards may be a potential source of food contamination as they can release chemicals (intentionally added or not), especially recycled paperboards. In this study, we assessed the in vitro genotoxicity of food contact paperboard samples from a manufacturer, collected at the beginning and at the end of a recycling production chain. Samples were extracted in water to mimic a wet food contact. Different genotoxic endpoints were evaluated in two human hepatic cell lines (HepG2 and HepaRG) using bioassays: γH2AX and p53 activation, primary DNA damage with the comet assay and micronucleus formation. We found that the samples from the beginning and the end of the production chain induced, with the same potency, γH2AX and p53-ser15 activation and DNA damage with the comet assay. The micronucleus assay was negative with the paperboard extract from the beginning of the chain, whereas positive data were observed for the end paperboard extract. Our results indicate that samples from recycled food contact paperboard can induce in vitro genotoxic effects in our experimental conditions.
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Soumis le : lundi 30 octobre 2017 - 09:23:07
Dernière modification le : lundi 21 novembre 2022 - 03:52:54



Emilie Souton, Isabelle Séverin, Ludovic Le Hegarat, Kevin Hogeveen, Abdulhadi Aljawish, et al.. Genotoxic effects of food contact recycled paperboard extracts on two human hepatic cell lines.. Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment, 2018, 35 (1), pp.159-170. ⟨10.1080/19440049.2017.1397774⟩. ⟨hal-01625970⟩



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