Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BMC Research Notes Année : 2017

Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger

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Résumé

OBJECTIVE: We conducted a parallel evaluation of the diagnostic accuracy of VIKIA® Rota-Adeno, a rapid diagnostic test (RDT) and Premier™ Rotaclone® an enzyme immunoassay (EIA) using reverse transcription polymerase chain reaction (RT-PCR) as the reference standard. The study was part of a rotavirus surveillance project in Niger. RESULTS: The sensitivity of both tests was 80.7%. After exclusion of one indeterminate result by visual reading, the specificity of the Premier™ Rotaclone® was 100% by visual or optical density readings and that of VIKIA® Rota-Adeno test was 95.5%. Inter-reader agreement was excellent for both tests (kappa = 1). Our results showed almost similar performance of the EIA and RDT when compared to RT-PCR. Hence, the VIKIA® Rota-Adeno could be a good alternative for use in peripheral health centres where laboratory capacity is limited.

Dates et versions

hal-01644851 , version 1 (22-11-2017)

Identifiants

Citer

Adamou Lagare, Aissatou Moumouni, Jérôme Kaplon, Céline Langendorf, Pierre Pothier, et al.. Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger. BMC Research Notes, 2017, 10 (1), pp.505. ⟨10.1186/s13104-017-2832-1⟩. ⟨hal-01644851⟩
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