Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger

Abstract : OBJECTIVE: We conducted a parallel evaluation of the diagnostic accuracy of VIKIA® Rota-Adeno, a rapid diagnostic test (RDT) and Premier™ Rotaclone® an enzyme immunoassay (EIA) using reverse transcription polymerase chain reaction (RT-PCR) as the reference standard. The study was part of a rotavirus surveillance project in Niger. RESULTS: The sensitivity of both tests was 80.7%. After exclusion of one indeterminate result by visual reading, the specificity of the Premier™ Rotaclone® was 100% by visual or optical density readings and that of VIKIA® Rota-Adeno test was 95.5%. Inter-reader agreement was excellent for both tests (kappa = 1). Our results showed almost similar performance of the EIA and RDT when compared to RT-PCR. Hence, the VIKIA® Rota-Adeno could be a good alternative for use in peripheral health centres where laboratory capacity is limited.
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01644851
Contributeur : Pam - Université de Bourgogne <>
Soumis le : mercredi 22 novembre 2017 - 16:13:38
Dernière modification le : jeudi 20 septembre 2018 - 10:10:07

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Adamou Lagare, Aissatou Moumouni, Jérôme Kaplon, Céline Langendorf, Pierre Pothier, et al.. Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger. BMC Research Notes, BioMed Central, 2017, 10 (1), 〈10.1186/s13104-017-2832-1〉. 〈hal-01644851〉

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