Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells

Etienne Morel; Sara Ghezzal; Geraldine Lucchi; Caroline Truntzer; Jean-Paul Pais de Barros; Françoise Simon-Plas; Sylvie Demignot; Chieko Mineo; Philip W Shaul; Armelle Leturque; Monique Rousset; Véronique Carriere

doi:10.1016/j.bbalip.2017.11.009

Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells

Etienne Morel ¹ Sara Ghezzal ¹ Geraldine Lucchi ² Caroline Truntzer ² Jean-Paul Pais de Barros ^{3, 4} Françoise Simon-Plas ⁵ Sylvie Demignot ¹ Chieko Mineo ⁶ Philip W Shaul ⁶ Armelle Leturque ¹ Monique Rousset ¹ Véronique Carriere ^{1, *}

* Auteur correspondant

1 CRC - Centre de Recherche des Cordeliers

2 CLIPP - Plate-forme Protéomique CLIPP - Clinical and Innovation Proteomic Platform [Dijon]

FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies, ICMUB - Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon]

3 LAP - Plateforme Lipidomique [Dijon]

LNC - Lipides - Nutrition - Cancer (U866), IFR100 - Structure fédérative de recherche Santé-STIC, CHU Dijon - Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand

4 LNC - Lipides - Nutrition - Cancer [Dijon - U1231]

5 Agroécologie [Dijon]

6 Center for Pulmonary and Vascular Biology : Department of Pediatrics [University of Texas, Southwestern Medical Center, Dallas]

Abstract : Scavenger receptor Class B type 1 (SR-B1) is a lipid transporter and sensor. In intestinal epithelial cells, SR-B1-dependent lipid sensing is associated with SR-B1 recruitment in raft-like/ detergent-resistant membrane domains and interaction of its C-terminal transmembrane domain with plasma membrane cholesterol. To clarify the initiating events occurring during lipid sensing by SR-B1, we analyzed cholesterol trafficking and raft-like domain composition in intestinal epithelial cells expressing wild-type SR-B1 or the mutated form SR-B1-Q445A, defective in membrane cholesterol binding and signal initiation. These features of SR-B1 were found to influence both apical cholesterol efflux and intracellular cholesterol trafficking from plasma membrane to lipid droplets, and the lipid composition of raft-like domains. Lipidomic analysis revealed likely participation of d18:0/16:0 sphingomyelin and 16:0/0:0 lysophosphatidylethanolamine in lipid sensing by SR-B1. Proteomic analysis identified proteins, whose abundance changed in raft-like domains during lipid sensing, and these included molecules linked to lipid raft dynamics and signal transduction. These findings provide new insights into the role of SR-B1 in cellular cholesterol homeostasis and suggest molecular links between SR-B1-dependent lipid sensing and cell cholesterol and lipid droplet dynamics.

Keywords : scavenger receptor cholesterol lipid raft sphingolipid lipid trafficking

Type de document :

Article dans une revue

Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, Elsevier, 2018, 1863 (2), pp.199-211. 〈http://www.sciencedirect.com/science/article/pii/S1388198117302330〉. 〈10.1016/j.bbalip.2017.11.009〉

Domaine :

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire

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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01661856
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : mercredi 13 décembre 2017 - 11:48:42
Dernière modification le : mercredi 21 mars 2018 - 18:57:52

Etienne Morel, Sara Ghezzal, Geraldine Lucchi, Caroline Truntzer, Jean-Paul Pais de Barros, et al.. Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, Elsevier, 2018, 1863 (2), pp.199-211. 〈http://www.sciencedirect.com/science/article/pii/S1388198117302330〉. 〈10.1016/j.bbalip.2017.11.009〉. 〈hal-01661856〉

Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells

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Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells

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