Calcein+/PI- as an early apoptotic feature in Leishmania

Louise Basmaciyan 1, 2 Nadine Azas 2 Magali Casanova 2, *
* Auteur correspondant
1 VAlMiS - Vin Aliment Microbiologie et Stress
PAM - Procédés Alimentaires et Microbiologiques, PAM - Procédés Alimentaires et Microbiologiques [Dijon]
Abstract : Although leishmaniases are responsible for high morbidity and mortality all over the world, no really satisfying treatment exists. Furthermore, the corresponding parasite Leishmania undergoes a very characteristic form of programmed cell death. Indeed, different stimuli can induce morphological and biochemical apoptotic-like features. However, the key proteins involved in mammal apoptosis, such as caspases and death receptors, are not encoded in the genome of this parasite. Currently, little is known about Leishmania apoptosis, notably owing to the lack of specific tools for programmed cell death analysis in these parasites. Furthermore, there is a need for a better understanding of Leishmania programmed cell death in order (i) to better understand the role of apoptosis in unicellular organisms, (ii) to better understand apoptosis in general through the study of an ancestral eukaryote, and (iii) to identify new therapeutic targets against leishmaniases. To advance understanding of apoptosis in Leishmania, in this study we developed a new tool based on the quantification of calcein and propidium iodide by flow cytometry. This double labeling can be employed to distinguish early apoptosis, late apoptosis and necrosis in Leishmania live cells with a very simple and rapid assay. This paper should, therefore, be of interest for people working on Leishmania and related parasites.
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PLoS ONE, Public Library of Science, 2017, 12 (11), 〈10.1371/journal.pone.0187756〉
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01666227
Contributeur : Pam - Université de Bourgogne <>
Soumis le : lundi 18 décembre 2017 - 10:42:22
Dernière modification le : vendredi 8 juin 2018 - 14:50:25

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Louise Basmaciyan, Nadine Azas, Magali Casanova. Calcein+/PI- as an early apoptotic feature in Leishmania. PLoS ONE, Public Library of Science, 2017, 12 (11), 〈10.1371/journal.pone.0187756〉. 〈hal-01666227〉

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