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Article dans une revue
A Thermophilic Tetramolecular G-Quadruplex/Hemin DNAzyme
Abstract : The quadruplex-based DNAzyme system is one of the most useful artificial enzymes or catalysts; their unique properties make them reliable alternatives to proteins for performing catalytic transformation. The first prototype of a thermally stable DNAzyme system is presented. This thermophilic DNAzyme is capable of oxidizing substrates at high temperatures (up to 95 degrees C) and long reaction times (up to 18 h at 75 degrees C). The catalytic activity of the DNAzymes were investigated with the standard peroxidase-mimicking oxidation of 2,2'-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS) by H2O2. The step-by-step design of this unique heat-activated G-quadruplex/hemin catalyst, including the modification of adenines at both ends of G-tracts, the choice of cation, and its concentration for DNAzyme stabilization, is described. This work investigates thoroughly the molecular basis of these catalytic properties and provides an example of an industrially relevant application.
https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01680478
Contributeur : Icmub - Université de Bourgogne <>
Soumis le : mercredi 10 janvier 2018 - 17:29:38
Dernière modification le : vendredi 17 juillet 2020 - 20:32:02
Contributeur : Icmub - Université de Bourgogne <>
Soumis le : mercredi 10 janvier 2018 - 17:29:38
Dernière modification le : vendredi 17 juillet 2020 - 20:32:02