A Thermophilic Tetramolecular G-Quadruplex/Hemin DNAzyme

Yuehua Guo 1 Jielin Chen 1 Mingpan Cheng 2 David Monchaud 3 Jun Zhou 1, * Huangxian Ju 1, *
* Auteur correspondant
1 State Key Laboratory of Analytical Chemistry for Life Science [School of Chemistry and Chemical Engineering, Nanjing University]
Nanjing University of Science and Technology [Nanjing]
2 State Key Laboratory of Catalysis [Dalian Institute of Chemical Physics, Chinese Academy of Sciences]
Dalian Institute of Chemical Physics - Chinese Academy of Sciences
3 ICMUB - Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon]
Abstract : The quadruplex-based DNAzyme system is one of the most useful artificial enzymes or catalysts; their unique properties make them reliable alternatives to proteins for performing catalytic transformation. The first prototype of a thermally stable DNAzyme system is presented. This thermophilic DNAzyme is capable of oxidizing substrates at high temperatures (up to 95 degrees C) and long reaction times (up to 18 h at 75 degrees C). The catalytic activity of the DNAzymes were investigated with the standard peroxidase-mimicking oxidation of 2,2'-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS) by H2O2. The step-by-step design of this unique heat-activated G-quadruplex/hemin catalyst, including the modification of adenines at both ends of G-tracts, the choice of cation, and its concentration for DNAzyme stabilization, is described. This work investigates thoroughly the molecular basis of these catalytic properties and provides an example of an industrially relevant application.
Keywords : DNAzyme G-quadruplexes hemin peroxidase activity thermophilicity peroxidase-mimicking dnazymes activity enhancement dna specificity oxidation insights complex porphyrin kinetics aptamers
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01680478
Contributeur : Icmub - Université de Bourgogne <>
Soumis le : mercredi 10 janvier 2018 - 17:29:38
Dernière modification le : vendredi 8 juin 2018 - 14:50:12

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Yuehua Guo, Jielin Chen, Mingpan Cheng, David Monchaud, Jun Zhou, et al.. A Thermophilic Tetramolecular G-Quadruplex/Hemin DNAzyme. Angewandte Chemie International Edition, Wiley-VCH Verlag, 2017, 56 (52), pp.16636 - 16640. ⟨10.1002/anie.201708964⟩. ⟨hal-01680478⟩

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