Potential added value of a RT-qPCR method of SOX 11 expression, in the context of a multidisciplinary diagnostic assessment of B cell malignancies

Julien Magne 1 Alizée Jenvrin 2 Adrien Chauchet 3 Olivier Casasnovas 4 Anne Donzel 1 Laurence Jego 1 Bernard Aral 1 Julien Guy 1 Nathalie Nadal 1 Dewi Vernerey 5 Patrick Callier 1 Francine Garnache-Ottou 2 Christophe Ferrand 2, 6, *
* Auteur correspondant
1 LNC - Lipides - Nutrition - Cancer (U866)
2 Inserm UMR 1098
3 Service d'hématologie
4 Service d'Hématologie Clinique (CHU de Dijon)
5 CEF2P / CARCINO - Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181)
6 Etablissement Français du Sang (EFS) - Bourgogne Franche-Comté
Abstract : Background Expression of SRY [sex-determining region Y]-box11 (SOX11) is specific to mantle cell lymphoma (MCL) and contributes, in conjunction with immunoglobulin variable heavy chain gene mutation status, to the identification of two forms of this disease. Methods The aim of this report was firstly, to design an easy and suitable RT-qPCR method to quantify SOX11 mRNA expression in mantle cell lymphoma and other B cell malignancies with the proper reference gene; secondly, to define the best threshold of relative quantity of SOX11 mRNA in order to reach the best compromise between sensitivity and specificity. Results For best discrimination of MCL and non-MCL groups we determined an area under the curve (AUC) of 0.9750 and a threshold of 1.76 with 100% sensitivity and 88% specificity. AUC and threshold values of respectively 0.91/1.346 [87% sensitivity, 80% specificity] and 0.9525/1.7120 [100% sensitivity, 88% specificity] for GAPDH and RPLP0 respectively denote that the RPLP0 reference gene alone is sufficient for PCR housekeeping gene. Conclusion This work describes an RT-qPCR assay for SOX11 expression in order to better characterize MCL at diagnosis. Further studies on larger cohorts are needed to evaluate this molecular tool, especially for the follow-up of minimal residual disease.
Keywords : Mantle cell lymphoma ROC curve analysis-threshold RT-qPCR-reference genes SOX11
Type de document :
Article dans une revue
Liste complète des métadonnées
https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01731294
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : mercredi 14 mars 2018 - 09:38:50
Dernière modification le : mercredi 7 août 2019 - 15:12:23

Lien texte intégral

openaccess

Identifiants

Collections

UNIV-BOURGOGNE | UNIV-FCOMTE | LNC-UMR866 | ENSBANA | AGROSUP-DIJON | LNC-SAPHIHR

Citation

Julien Magne, Alizée Jenvrin, Adrien Chauchet, Olivier Casasnovas, Anne Donzel, et al.. Potential added value of a RT-qPCR method of SOX 11 expression, in the context of a multidisciplinary diagnostic assessment of B cell malignancies. Experimental Hematology & Oncology, BMC, 2018, 7 (1), pp.5. ⟨10.1186/s40164-018-0097-6⟩. ⟨hal-01731294⟩

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

122

Contact

Informations

CCSD