Strategies to improve carotene entry into cells of Yarrowia lipolytica in a goal of encapsulation

Abstract : Yeasts are good materials for encapsulation of actives, but, as they are preformed capsules, they have to be loaded. Our goal was to load cells with a big and very hydrophobic molecule, beta-carotene (LogP approximate to 15). In classical conditions, the entry of this compound into cells is negligible and we have worked on ways to favor the entry through the cell wall. Carotene was prepared at 0.3-3 g L-1 in solvents to improve solubility and alter the cell wall structure. After a 3 h-incubation of cells with this solution, with the apolar hexane, the entry was increased to 95 mu g g(-1) cell wet weight but the monopolar chloroform was much more efficient (220 mu g g(-1) cell wet weight). However, increasing the carotene concentration in chloroform increased the adhesion of carotene on the cell wall (over 900 mu g g(-1) cell wet weight) but did not increase the amount into the cell. The use of ultrasound (6-min-treatment at 50% and an output control of 4) as a green physical treatment increased significantly the result for beta-carotene encapsulation (852 mu g g(-1) cell wet weight).
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01894665
Contributeur : Pam - Université de Bourgogne <>
Soumis le : vendredi 12 octobre 2018 - 16:06:07
Dernière modification le : vendredi 30 novembre 2018 - 15:44:28

Identifiants

Citation

Bao Ngoc Pham-Hoang, Cynthia Romero-Guido, Hanh Phan-Thi, Yves Waché. Strategies to improve carotene entry into cells of Yarrowia lipolytica in a goal of encapsulation. Journal of Food Engineering, Elsevier, 2018, 224, pp.88 - 94. ⟨10.1016/j.jfoodeng.2017.12.029⟩. ⟨hal-01894665⟩

Partager

Métriques

Consultations de la notice

40