Cell Death Mechanisms Induced by Photo-Oxidation Studied at the Cell Scale in the Yeast Saccharomyces cerevisiae

Cédric Grangeteau 1 Florine Lepinois 2 Pascale Winckler 1, 3 Jean-Marie Perrier-Cornet 1, 3 Sébastien Dupont 1 Laurent Beney 1, *
* Auteur correspondant
1 PMB - Procédés Microbiologiques et Biotechnologiques
PAM - Procédés Alimentaires et Microbiologiques, PAM - Procédés Alimentaires et Microbiologiques [Dijon]
2 PAM - Procédés Alimentaires et Microbiologiques [Dijon]
3 DImaCell - Dispositif Inter-régional d'Imagerie Cellulaire [Dijon]
PAM - Procédés Alimentaires et Microbiologiques, IBCT (ex IFR133) - Ingénierie et biologie cellulaire et tissulaire
Abstract : Blue light (400-430 nm) is known to induce lethal effects in some species of fungi by photo-oxidation caused by the excitation of porphyrins but the mechanisms involved remain poorly understood. In this work, we exposed the yeast Saccharomyces cerevisiae to a high density light flux with two-photon excitation (830 nm equivalent to a one-photon excitation around 415 nm) and used quasi real-time visualization with confocal microscopy to study the initiation and dynamics of photo-oxidation in subcellular structures. Our results show that the oxidation generated by light treatments led to the permeabilization of the plasma membrane accompanied by the sudden expulsion of the cellular content, corresponding to cell death by necrosis. Moreover, excitation in the plasma membrane led to very fast oxidation and membrane permeabilization (< 60 s) while excitation at the center of the cell did not induce permeabilization even after a period exceeding 600 s. Finally, our study shows that the relationship between the laser power used for two-photon excitation and the time required to permeabilize the plasma membrane was not linear. Thus, the higher the power used, the lower the energy required to permeabilize the plasma membrane. We conclude that fungal destruction can be generated very quickly using a high density light flux. Better knowledge of the intracellular processes and the conditions necessary to induce necrosis should make it possible in the future to improve the efficiency of antimicrobial strategies photo-oxidation-based.
Keywords : singlet oxygen violet 405-nm light blue light High power light two-photon yeast porphyrins photo-oxidation lipid-peroxidation nm light blue-light inactivation visible-light candida-albicans photodynamic therapy staphylococcus-aureus
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01950369
Contributeur : Pam - Université de Bourgogne <>
Soumis le : lundi 10 décembre 2018 - 17:32:09
Dernière modification le : vendredi 18 octobre 2019 - 15:21:43

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Cédric Grangeteau, Florine Lepinois, Pascale Winckler, Jean-Marie Perrier-Cornet, Sébastien Dupont, et al.. Cell Death Mechanisms Induced by Photo-Oxidation Studied at the Cell Scale in the Yeast Saccharomyces cerevisiae. Frontiers in Microbiology, Frontiers Media, 2018, 9, ⟨10.3389/fmicb.2018.02640⟩. ⟨hal-01950369⟩

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