Study and optimization of freeze-drying cycles of a model probiotic strain

Pierre Verlhac (1) , Séverine Vessot-Crastes (1) , Ghania Degobert (1) , Claudia Cogné (1) , Julien Andrieu (1) , Laurent Beney (2) , Patrick Gervais (2)

1 LAGEPP - Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique
2 PAM - Procédés Alimentaires et Microbiologiques [Dijon]

This work is based on the experimental study of the freeze-drying process to understand the impact of numerous factors on the survival rates of a model probiotic strain of Lactobacillus casei type. With the aim to find out if cell density in the matrix and survival rates are linked, we have studied the location of the cells after freeze drying inside a porous matrix composed of a lactose basis with a polymer, the polyvinylpyrrolidone (PVP) in various amounts. The best survival rate were obtained at slow freezing rate for a formulation containing 5% (m/V) oflactose and 5% (m/V) of PVP.

Mots clés: Freeze-Drying; Freezing; Probiotics; L. Casei ATCC 393

Domaines: Sciences du Vivant [q-bio]; Ingénierie des aliments

Pierre Verlhac, Séverine Vessot-Crastes, Ghania Degobert, Claudia Cogné, Julien Andrieu, et al.. Study and optimization of freeze-drying cycles of a model probiotic strain. 21st International Drying Symposium, Universitat politècnica de valència, Sep 2018, Valence, Spain. ⟨hal-02055897⟩