Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Identification and transcriptional profile of Lactobacillus paracasei genes involved in the response to desiccation and rehydration

Aurore Palud 1, * Karima Salem 1, 2 Jean-François Cavin 3 Laurent Beney 3 Hélène Licandro 3, *
* Auteur correspondant
3 PMB - Procédés Microbiologiques et Biotechnologiques
PAM - Procédés Alimentaires et Microbiologiques, PAM - Procédés Alimentaires et Microbiologiques [Dijon]
Abstract : Lactobacillus paracasei is able to persist in a variety of natural and technological environments despite physico-chemical perturbations, in particular alternations between desiccation and rehydration. However, the way in which it adapts to hydric fluctuations and the genetic determinants involved are not clearly understood. To identify the genes involved in adaptation to desiccation, an annotated library of L. paracasei random transposon mutants was screened for viability after desiccation (25% relative humidity, 25 °C). We found 16 genes that have not been described as being involved in this response. Most of them are linked to either the transport of molecules or to cell wall structure and function. Our screening also identified genes encoding DNA related enzymes and an alarmone necessary for L. paracasei survival. Subsequently, the expression of the identified genes was measured at five stages of the dehydration-rehydration process to decipher the chronology of genetic mechanisms. They were classified into four different transcriptional profiles: genes upregulated during both desiccation and rehydration phases, genes upregulated during the desiccation phase only, genes downregulated during both desiccation and rehydration and genes downregulated only during the rehydration stage. Thus, genetic response to hydric fluctuations seems to occur during desiccation and can continue or not during rehydration. The genes identified should contribute to improve the stabilization of Lactobacillus starters in dry state.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-univ-bourgogne.archives-ouvertes.fr/hal-02310935
Contributeur : Pam - Université de Bourgogne <>
Soumis le : jeudi 10 octobre 2019 - 15:25:58
Dernière modification le : mardi 16 juin 2020 - 08:06:02

Lien texte intégral

Identifiants

Citation

Aurore Palud, Karima Salem, Jean-François Cavin, Laurent Beney, Hélène Licandro. Identification and transcriptional profile of Lactobacillus paracasei genes involved in the response to desiccation and rehydration. Food Microbiology, Elsevier, 2020, 85, pp.103301. ⟨10.1016/j.fm.2019.103301⟩. ⟨hal-02310935⟩

Partager

Métriques

Consultations de la notice

74