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Article dans une revue

Analytical combinations to evaluate the macromolecular composition of extracellular substances (ECS) from Lactobacillus plantarum cell culture media

Peio Elichiry-Ortiz 1 Pauline Maes 2 Stéphanie Weidmann 1 Dominique Champion 1 Christian Coelho 1, *
* Auteur correspondant
2 CLIPP - Plate-forme Protéomique CLIPP - Clinical and Innovation Proteomic Platform [Dijon]
FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174), ICMUB - Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon]
Abstract : Sugar-enriched media are used to produce extracellular substances (ECS) by Lactobacillus plantarum WCSF1, with a focus on growing stages and carbon source substrates. Combination of size exclusion chromatography and ATR-FTIR spectroscopy provides physicochemical patterns of bulk ECS produced along culture growing time. Secreted biopolymers present polydisperse and high molecular weight distributions, with significant amounts of carbohydrates and proteins. Results, supported by a multivariate statistical analysis, enable to differentiate the macromolecular content of bacterial ECS along the growing stages regardless of the growing media, highlighting a higher production of proteinaceous materials compared to polysaccharides. At the end of the exponential phase, common exoproteins were present in all the tested sugar-enriched media such as transglycosylases between 20 and 35 kDa, a muropeptidase at 36.9 kDa and a cell wall hydrolase. Additionally, L. plantarum WCFS1 secretes ECS with a greater diversity of proteins, when growing in the sucrose-enriched media. Graphical abstract.
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Article dans une revue
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-03016444
Contributeur : Pam - Université de Bourgogne <>
Soumis le : vendredi 20 novembre 2020 - 13:45:46
Dernière modification le : jeudi 26 novembre 2020 - 09:58:24

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Peio Elichiry-Ortiz, Pauline Maes, Stéphanie Weidmann, Dominique Champion, Christian Coelho. Analytical combinations to evaluate the macromolecular composition of extracellular substances (ECS) from Lactobacillus plantarum cell culture media. Analytical and Bioanalytical Chemistry, Springer Verlag, 2020, ⟨10.1007/s00216-020-03022-8⟩. ⟨hal-03016444⟩

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