PCR ITS-RFLP for Penicillium Species and Other Genera

Sandrine Rousseaux 1, * Michèle Guilloux-Benatier 1
* Auteur correspondant
1 IUVV Jules Guyot - Institut Universitaire de la Vigne et du Vin "Jules Guyot"
Abstract : Among numerous molecular methodologies developed for highly specific identification of filamentous fungi isolates, here we describe restriction digestion analysis of the ITS products as an easy method to identify isolates of filamentous fungi. This technique is a rapid and reliable method appropriate for routine identification of filamentous fungi. This can be used to screen large numbers of isolates from various environments in a short time. The use of different endonucleases allowed generating individual restriction profiles. The individual profiles obtained were combined into composite restriction patterns characteristic of a species. Eleven different genera can be differentiated and among them 41 different species.
Keywords : Filamentous fungi PCR ITS-RFLP Identification Composite profiles
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01511211
Contributeur : Pam - Université de Bourgogne <>
Soumis le : jeudi 20 avril 2017 - 15:23:17
Dernière modification le : lundi 2 juillet 2018 - 16:26:04

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Sandrine Rousseaux, Michèle Guilloux-Benatier. PCR ITS-RFLP for Penicillium Species and Other Genera. Methods in Molecular Biology, Humana Press/Springer Imprint, 2017, Mycotoxigenic Fungi : Methods and Protocols [Part IV], 1542, pp.321-333. ⟨10.1007/978-1-4939-1658-0⟩. ⟨hal-01511211⟩

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