Chitosan nanoparticles plus quercetin suppress the oxidative stress, modulate DNA fragmentation and gene expression in the kidney of rats fed ochratoxin A-contaminated diet.

Abstract : This study aimed to evaluate the protective role of chitosan nanoparticles (COS-NPs) singly or plus quercetin (Q) against OTA-induced oxidative stress and renal genotoxicity. Twelve groups of male Sprague-Dawley rats were treated orally for 3 weeks included the control group, animals fed OTA-contaminated diet (3 mg/kg diet); COS-NPs-treated groups at low (140 mg/kg b.w.) or high (280 mg/kg b.w.) dose, Q-treated group (50 mg/kg b.w.), Q plus low or high dose of COS-NPs-treated groups and OTA plus Q and/or COS-NPs at the two tested doses-treated groups. The results indicated that COS-NPs were roughly rod in shape with average particle size of 200 nm and zeta potential 31.4 ± 2.8 mV. Animals fed OTA-contaminated diet showed significant changes in serum biochemical parameters, increase kidney MDA and DNA fragmentation and decreased GPx and SOD gene expression accompanied with severe histological changes. Q and/or COS-NPs at the two tested doses induced significant improvements in all tested parameters and succeeded to overcome these effects especially in the animals treated with Q plus the high dose of COS-NPs. It could be concluded that COS-NPs are promise candidate to enhance the antioxidant effect of Q and protect against the nephrotoxicity of OTA in high endemic areas.
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01552979
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : lundi 3 juillet 2017 - 12:47:58
Dernière modification le : vendredi 8 juin 2018 - 14:50:25

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Mosaad A Abdel-Wahhab, Abdulhadi Aljawish, Aziza A El-Nekeety, Sekena H Abdel-Aziem, Nabila S Hassan. Chitosan nanoparticles plus quercetin suppress the oxidative stress, modulate DNA fragmentation and gene expression in the kidney of rats fed ochratoxin A-contaminated diet.. Food and Chemical Toxicology, Elsevier, 2017, 99, pp.209-221. 〈http://www.sciencedirect.com/science/article/pii/S0278691516304550〉. 〈10.1016/j.fct.2016.12.002〉. 〈hal-01552979〉

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