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Shigella entry unveils a calcium/calpain-dependent mechanism for inhibiting sumoylation

Abstract : Disruption of the sumoylation/desumoylation equilibrium is associated with several disease states such as cancer and infections, however the mechanisms regulating the global SUMO balance remain poorly defined. Here, we show that infection by Shigella flexneri, the causative agent of human bacillary dysentery, switches off host sumoylation during epithelial cell infection in vitro and in vivo and that this effect is mainly mediated by a calcium/calpain-induced cleavage of the SUMO E1 enzyme SAE2, thus leading to sumoylation inhibition. Furthermore, we describe a mechanism by which Shigella promotes its own invasion by altering the sumoylation state of RhoGDI alpha, a master negative regulator of RhoGTPase activity and actin polymerization. Together, our data suggest that SUMO modification is essential to restrain pathogenic bacterial entry by limiting cytoskeletal rearrangement induced by bacterial effectors. Moreover, these findings identify calcium-activated calpains as powerful modulators of cellular sumoylation levels with potentially broad implications in several physiological and pathological situations.
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https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01687185
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Soumis le : mercredi 7 février 2018 - 14:09:47
Dernière modification le : mercredi 23 septembre 2020 - 04:35:14

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Pierre Lapaquette, Sabrina Fritah, Nouara Lhocine, Alexandra Andrieux, Giulia Nigro, et al.. Shigella entry unveils a calcium/calpain-dependent mechanism for inhibiting sumoylation. eLife, eLife Sciences Publication, 2017, pp.e27444. ⟨10.7554/eLife.27444⟩. ⟨hal-01687185⟩

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