Accéder directement au contenu Accéder directement à la navigation
Communication dans un congrès

Sperm imprinting integrity in seminoma patients?

Abstract : Background Testicular germ cell tumor such as seminoma is strongly associated with male reproductive problems commonly associated with the alteration of sperm parameters as described in testicular dysgenesis syndrome. Interestingly, numerous studies have reported that the precursor of germ cell cancer, germ cell neoplasia in situ (GCNIS), present similarities to fetal gonocytes, specifically characterized by global DNA hypomethylation particularly on imprinting sequences. These disorders may have a common origin derived from perturbations of embryonal programming during fetal development. Presently, there is no available information concerning the sperm DNA methylation patterns of testicular cancer patients. For the first time, we evaluated the sperm imprinting of seminoma patients. A total of 92 cryopreserved sperm samples were included, 31 before seminoma treatment (S): 23 normozoospermic (SN) and 8 oligozoospermic (SO) and 61 sperm controls samples: 31 normozoospermic (N) and 30 oligozoospermic (O). DNA methylation levels of seven differentially methylated regions (DMRs) of imprinted genes [H19/IGF2: IG-DMR (CTCF3 and CTCF6 of H19 gene); IGF2-DMRs (DMR0 and DMR2); MEG3/DLK1:IG-DMR; SNURF:TSS-DMR; KCNQ1OT1:TSS-DMR] were assessed by pyrosequencing. All comparative analyses were adjusted for age. Results Comparisons of sperm DNA methylation levels between seminoma (S) and normozoospermic (N) samples showed a significant difference for the SNURF sequence (p = 0.017), but after taking into account the sperm parameters, no difference was observed. However, we confirmed a significant association between oligozoospermia (O) and imprinting defects for H19/IGF2-CTCF6 (p = 0.001), MEG3/DLK1 (p = 0.017), IGF2-DMR2 (p = 0.022), and SNURF (p = 0.032) in comparison with control groups (N). Conclusions This study highlights the high risk of sperm imprinting defects in cases of oligozoospermia and shows for the first time that seminoma patients with normal spermatogenesis present sperm imprinting integrity. These data suggest a low probability of the involvement of a common imprinting defect in fetal cells leading to both TGCT and subfertility.
Type de document :
Communication dans un congrès
Liste complète des métadonnées

https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01936132
Contributeur : Lnc - Université de Bourgogne <>
Soumis le : mardi 27 novembre 2018 - 11:41:33
Dernière modification le : mercredi 28 novembre 2018 - 01:16:30

Identifiants

  • HAL Id : hal-01936132, version 1

Citation

Céline Bruno, Oxana Blagoskonov, Julie Barberet, Magali Guilleman, Sandrine Daniel, et al.. Sperm imprinting integrity in seminoma patients?. 34th Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE), Jun 2018, Barcelone, Spain. pp.O-076. ⟨hal-01936132⟩

Partager

Métriques

Consultations de la notice

47