Size measuring techniques as tool to monitor pea proteins intramolecular crosslinking by transglutaminase treatment

Attaf Djoullah (1, 2) , Ghali Krechiche (1, 2) , Florence Husson (1) , Rémi Saurel (1)

1 PAM - Procédés Alimentaires et Microbiologiques
2 Department of Food Technologies [Alger]

In this work, techniques for monitoring the intramolecular transglutaminase cross-links of pea proteins, based on protein size determination, were developed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis profiles of transglutaminase-treated low concentration (0.01% w/w) pea albumin samples, compared to the untreated one (control), showed a higher electrophoretic migration of the major albumin fraction band (26 kDa), reflecting a decrease in protein size. This protein size decrease was confirmed, after DEAE column purification, by dynamic light scattering (DLS) where the hydrodynamic radius of treated samples appears to be reduced compared to the control one.

Mots clés: Microbial transglutaminase; Pea albumin; Intramolecular crosslinks; Electrophoresis; Dynamic light scattering; Amino-acid-composition; Patterns

Domaines: Ingénierie des aliments

Attaf Djoullah, Ghali Krechiche, Florence Husson, Rémi Saurel. Size measuring techniques as tool to monitor pea proteins intramolecular crosslinking by transglutaminase treatment. Food Chemistry, 2016, 190, pp.197 - 200. ⟨10.1016/j.foodchem.2015.05.091⟩. ⟨hal-01484094⟩